A multi-omics resource of B cell response to SARS-CoV-2 mRNA vaccine in naive individuals
We used SARS-CoV-2 mRNA-1273 vaccination as a model to fill the knowledge gap in dynamics of human Class Switch Recombination (CSR) during primary responses by investigating the evolution of B cells with particular focus on B cell receptor (BCR) features, isotype usage, as well as the cellular dynamics through time.
We profiled both vaccine-derived, antigen-specific B cells, as well as their non-vaccine-derived counterparts from blood samples using a combination of single-cell transcriptomics (10X Chromium) and flow cytometry. Extensive BCR sequences were collected using a long-read sequencing approach to sample the bulk BCR repertoire as well as 10X Chromium-based single-cell BCR sequencing. Antigen-specific antibody titres were measured in the serum and compared to saliva samples.
To facilitate interactive interrogation of this data resource, we have prepared this webpage to facilitate inspection of our data using online applications to query our scRNA-seq data, as well as various measurements we collected from this study. Please navigate to the following pages to interact with our dataset!
